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Inducible CRISPR-Cas9 improves the precision of genome editing in rice

September 19th, 2019 / ISAAA, US

DNA delivery into tissue cultures is a simple method for expressing CRISPR-Cas9 and creating genome edits in the plants. This approach, however, allows strong doses of CRISPR-Cas9 to persist far beyond the incidence of targeting i.e. until genetic segregation of the Cas9 gene.

Although precision is an inherent quality of CRISPR-Cas9, the strong persistent doses in numerous cells throughout the …