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Inducible CRISPR-Cas9 improves the precision of genome editing in rice

September 19th, 2019 / ISAAA, US

DNA delivery into tissue cultures is a simple method for expressing CRISPR-Cas9 and creating genome edits in the plants. This approach, however, allows strong doses of CRISPR-Cas9 to persist far beyond the incidence of targeting i.e. until genetic segregation of the Cas9 gene.

Although precision is an inherent quality of CRISPR-Cas9, the strong persistent doses in numerous cells throughout the …

Genome editing in agriculture: methods, applications, and governance

July 12th, 2018 / ISAAA, US

The Council for Agricultural Science and Technology (CAST) released an Issue Paper titled Genome Editing in Agriculture: Methods, Applications, and Governance. CAST explains that genome editing is a powerful new method that enables unprecedented control over genetic material and offers the opportunity to make rapid advances that influence agricultural practices. …

Where GM meets GE

May 21st, 2018 / Eureka Alert, AAAS, US

A new field trial of genetically modified Camelina, announced today by Defra, will be accompanied by one of the world’s first field trials of a genome-edited crop.
Genome edited (GE) crops will be sown in a field this month for the first time in the UK as part of an experimental …

Beyond CRISPR: A guide to the many other ways to edit a genome

August 11th, 2016 / Nature, UK

The CRISPR–Cas9 tool enables scientists to alter genomes practically at will. Hailed as dramatically easier, cheaper and more versatile than previous technologies, it has blazed through labs around the world, finding new applications in medicine and basic research.
But for all the devotion, CRISPR–Cas9 has its limitations. It is excellent at …